Test Code WBC White Blood Cell Count

Additional Codes

Cerner	NextGen
White Blood Cell Count	White Blood Cell Count
WBC

Alternate Name(s)

WBC

WBC Count

Leukocyte Count

Patient Preparation

None

Collection Instructions

Do not place samples on a mechanical rocker.

Specimen Requirements

Preferred Volume	12 x 75 mm tube EDTA-2K or EDTA-3K Lavender filled to capacity
Minimum Volume	1mL of whole blood in 12 x 75 mm tube EDTA-2K or EDTA-3K Lavender 160 μL of whole blood in microtube EDTA-2K or EDTA-3K Lavender
Container	12 x 75 mm tube EDTA-2K or EDTA-3K Lavender Microtube EDTA-2K or EDTA-3K Lavender
Stability	Sample stability at room temperature is 24 hours. Stored at 2-8^oC, EDTA blood samples with normal results may be analyzed up to 48 hours
Rejection Criteria	Clotted samples or those containing clots, fibrin strands, or platelet clumps. All specimens will be checked visually for obvious clots prior to sampling by the analyzer. Grossly hemolyzed samples. Samples drawn above an IV line.

Result Reporting and Reference Values

Reference Range

Units	Sex	Age	Reference Range
x10³/µL	M	0D-15D	5	--	30
		15D-30D	5	--	20
		30D-1Y	5	--	17.5
		1Y-10Y	4	--	13.5
		10Y-15Y	4.5	--	13
		15Y-150Y	4.8	--	10.5
	F	0D-15D	5	--	30
		15D-30D	5	--	20
		30D-1Y	5	--	17.5
		1Y-10Y	4.5	--	13.5
		10Y-15Y	4.5	--	13
		15Y-150Y	4	--	11

Critical Ranges

< 2,000 /µL
>50,000 /µL

Reflex Testing

Slide examination occurs when:
- Instrument parameter flags indicate abnormal cell populations, requiring a technologist to review slides to verify cell identity and abnormal morphology.
- Less than 2,000 cells/µL.
- Greater than 25,000 cells/µL

Limitations

None

Useful For/ Methodology

Sysmex XN, flow cytometry with a semiconductor laser

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