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Test Code VITD25OH 25-OH Vitamin D Total

Additional Codes

Cerner

NextGen

Vitamin D(25-OH), T

Vitamin D(25-OH), Total

25-OH Vitamin D, Total

 

Alternate Name(s)

Vitamin D

25-OH D

25-Hydroxy D

25OHVD

Vitamin D2 and D3

Ergocalciferol and Cholecalciferol

Methodology

Competitive Immunoassay

Patient Preparation

None

Collection Instructions

Standard phlebotomy practices.

Specimen Requirements

Container

Specimen Type

Tube

Serum

 or 

SST

 or  or 

 

Stability

Storage

Temperature

Stability

Room Temperature

18-28°C

Not recommended

Refrigerated

 2-8°C

≤ 7 days

Frozen

≤-20 °C

4 weeks

Thaw 1 time only

Rejection Criteria

Gross Hemolysis

Turbid Specimens

Result Reporting and Reference Values

Reported in ng/mL

Clinical Level

Range

Deficient

<20

Insufficient

20 - <30

Sufficient

30 -100

Potential Toxicity

>100

Reflex Testing

None

Limitations

  • Ortho reports a bias with the following:
    • Paricalcitol (Zemplar) at 24 ng/dL may cause a 10.5 ng/mL bias.
  • Ortho reports no significant effect with the following:
    • Bilirubin up to 30 mg/dL
    • Hemoglobin up to 200 mg/dL

Useful For

Vitamin D is a fat soluble steroid hormone that comes in two forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D is synthesized from cholesterol upon skin exposure to UVB sunlight or through dietary intake. Vitamin D is hydroxylated in the liver to form 25-OH Vitamin D which is further hydroxylated in the kidney to form the biologically active form, 1,25-(OH)2 Vitamin D. The active hormone is tightly regulated by plasma parathyroid hormone levels and serum calcium and phosphorous levels. The active form, 1,25-(OH)2 Vitamin D, increases the intestinal absorption of calcium and phosphorous, both are required for regulating bone metabolism. Recently, research has indicated that vitamin D deficiency may be linked to chronic diseases such as cancer (breast, colon and prostate), cardiovascular disease, osteoporosis, osteomalacia and several autoimmune diseases among others.