Test Code VITD25OH 25-OH Vitamin D Total
Additional Codes
Cerner |
NextGen |
Vitamin D(25-OH), T |
Vitamin D(25-OH), Total |
25-OH Vitamin D, Total |
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Alternate Name(s)
Vitamin D
25-OH D
25-Hydroxy D
25OHVD
Vitamin D2 and D3
Ergocalciferol and Cholecalciferol
Methodology
Competitive Immunoassay
Patient Preparation
None
Collection Instructions
Standard phlebotomy practices.
Specimen Requirements
Container |
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Stability |
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Rejection Criteria |
Gross Hemolysis Turbid Specimens |
Result Reporting and Reference Values
Reported in ng/mL
Clinical Level |
Range |
Deficient |
<20 |
Insufficient |
20 - <30 |
Sufficient |
30 -100 |
Potential Toxicity |
>100 |
Reflex Testing
None
Limitations
- Ortho reports a bias with the following:
- Paricalcitol (Zemplar) at 24 ng/dL may cause a 10.5 ng/mL bias.
- Ortho reports no significant effect with the following:
- Bilirubin up to 30 mg/dL
- Hemoglobin up to 200 mg/dL
Useful For
Vitamin D is a fat soluble steroid hormone that comes in two forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D is synthesized from cholesterol upon skin exposure to UVB sunlight or through dietary intake. Vitamin D is hydroxylated in the liver to form 25-OH Vitamin D which is further hydroxylated in the kidney to form the biologically active form, 1,25-(OH)2 Vitamin D. The active hormone is tightly regulated by plasma parathyroid hormone levels and serum calcium and phosphorous levels. The active form, 1,25-(OH)2 Vitamin D, increases the intestinal absorption of calcium and phosphorous, both are required for regulating bone metabolism. Recently, research has indicated that vitamin D deficiency may be linked to chronic diseases such as cancer (breast, colon and prostate), cardiovascular disease, osteoporosis, osteomalacia and several autoimmune diseases among others.