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Test Code HGB Hemoglobin

Additional Codes

Cerner

NextGen

Hemoglobin

Hemoglobin

HGB

 

Alternate Name(s)

HGB

Patient Preparation

None

Collection Instructions

Do not place samples on a mechanical rocker.

Specimen Requirements

Preferred Volume

12 x 75 mm tube EDTA-2K or EDTA-3K Lavender filled to capacity

Minimum Volume
  • 1mL of whole blood in 12 x 75 mm tube EDTA-2K or EDTA-3K Lavender
  • 160 μL of whole blood in microtube EDTA-2K or EDTA-3K Lavender

Container
  • 12 x 75 mm tube EDTA-2K or EDTA-3K Lavender


Microtube EDTA-2K or EDTA-3K Lavender

Stability
  • Sample stability at room temperature is 24 hours.
  • Stored at 2-8oC, EDTA blood samples with normal results may be analyzed up to 48 hours

Rejection Criteria
  • Clotted samples or those containing clots, fibrin strands, or platelet clumps.  All specimens will be checked visually for obvious clots prior to sampling by the analyzer.
  • Grossly hemolyzed samples.
  • Samples drawn above an IV line.

Result Reporting and Reference Values

Reference Ranges

Units

Sex

Age

Reference Range

gm/dL

All

0D-15D

12

--

23

All

15D-30D

10

--

20

All

30D-60D

11

--

14.5

All

60D-120D

10

--

14.5

All

120D-150D

10.5

--

14.5

All

150D-180D

10.8

--

14.5

M

180D-3Y

11

--

14.5

M

3Y-4Y

11.4

--

14.5

M

4Y-8Y

11.5

--

14.5

M

8Y-12Y

11.8

--

14.5

M

12Y-15Y

13

--

15

M

15Y-150Y

13.5

--

17.5

F

180D-2Y

11

--

14.5

F

2Y-3Y

11.4

--

14.5

F

3Y-7Y

11.5

--

14.5

F

7Y-11Y

11.8

--

14.5

F

11Y-12Y

12

--

14.5

F

12Y-120Y

11.7

--

15.7

 

Critical Ranges

Units

Sex

Age

Critical

gm/dL

All

0D-15D

<6

>24

All

15D-120Y

<6

>22

 

Reflex Testing

  • Slide examination occurs when:
    • HGB Less than 7 gm/dL.
    • HGB greater than 18 gm/dL for 12Years -150Years; >24gm/dL for 0-15 days; and >21 gm/dL for 15Days -30Days
    • Abnormal red blood cell quantitations.

  • Abnormal Heme Smear Path Review will be performed for:
    • Unexplained Hgb below 7 gm/dl if accompanied by abnormal RBC morphology or low or high MCV.
    • Unexplained presence of normoblasts during slide examination.
    • Any bizarre cells seen during slide examination.

Limitations

  • Delta check: Hgb change of > 2 gm/dL without explanation requires a rerun or redrawn specimen.
  • Abnormal paraproteins found in blood from patients with Multiple Myeloma can falsely increase the HGB.
  • HGB cannot be reported with turbid or lipemic specimens
  • WBC's greater than 100,000/µL may cause turbidity and falsely increase the hemoglobin value.
  • Severely icteric samples may falsely elevate the HGB value.

Useful For/ Methodology

Sysmex XN, conversion of SLS-hemoglobin and read photometrically

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