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HelpTest Code HEMMF Hematologic Specified FISH, Varies
Ordering Guidance
Consult with the laboratory before ordering this test.
This test is intended for instances when client-specified fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the ordering request. If targeted FISH probes are not included with this test order, test processing will be delayed and the test may be canceled by the laboratory.
Paraffin-embedded specimens and blood or bone marrow specimens submitted for evaluation of specific nonhematologic malignancies or congenital/hereditary testing must be ordered as MISCF / Miscellaneous Studies Using Chromosome-Specific Probes, FISH. If HEMMF is ordered in these situations, it will be canceled and MISCF ordered and performed as the appropriate test.
Necessary Information
1. A list of probes requested for analysis is required.
2. A reason for testing must be provided. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
3. A flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. Acceptable pathology reports include working drafts, preliminary pathology, or surgical pathology reports. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Acceptable:
Specimen Type: Whole Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Secondary ID
614265Useful For
Detecting specific chromosomal abnormalities in hematologic malignancies using client-specified probe set(s)
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for 1 probe set (2 individual fluorescence in situ hybridization [FISH] probes). Additional charges will be incurred for all reflex testing, if requested, or additional probe sets performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
This test is performed using client-specified FISH probes. Specific probes must be listed in the probe request field. Reflex probes can be performed when appropriate if specified in the order request field.
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Hematologic Specified FISHSpecimen Type
VariesSpecimen Minimum Volume
Bone marrow: 1 mL; Whole blood: 2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Fresh tissue | Reject |
Clinical Information
Fluorescence in situ hybridization using gene-specific probes and various probe strategies can help characterize chromosome abnormalities in hematologic malignancies for diagnostic, prognostic, and therapeutic purposes.
Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given fluorescence in situ hybridization probe set.
The absence of an abnormal clone does not exclude the presence of a neoplastic disorder.
Day(s) Performed
Monday through Friday
Report Available
9 to 12 daysSpecimen Retention Time
4 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271x2, 88275x1, 88291x1 - FISH Probe, Analysis, Interpretation; 1 probe set
88271x2, 88275x1 - FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HEMMF | Hematologic Specified FISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 614267 | Result Summary | 50397-9 |
| 614268 | Interpretation | 69965-2 |
| 614269 | Result Table | 93356-4 |
| 614270 | Result | 62356-1 |
| GC117 | Reason for Referral | 42349-1 |
| GC118 | Probes Requested | 78040-3 |
| GC119 | Specimen | 31208-2 |
| 614271 | Source | 31208-2 |
| 614272 | Method | 85069-3 |
| 614273 | Additional Information | 48767-8 |
| 614274 | Disclaimer | 62364-5 |
| 614275 | Released By | 18771-6 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HEMMB | Probe, Each Additional (HEMMF) | No, (Bill Only) | No |