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Test Code HDL High Density Lipoprotein Direct

Additional Codes

Cerner

NextGen

HDL Cholesterol

HDL Cholesterol

Methodology

Colorimetric (reflectance spectrophotometry).

Patient Preparation

12 hour fasting: Tests Requiring Fasting and Fasting Instructions

Collection Instructions

Standard phlebotomy practices.

Specimen Requirements

Container

Specimen Type

Tube

Lithium Heparin Plasma

PST

 or  or 

Serum

 or 

SST

 or  or 

 

Stability

Storage

Temperature

Stability

Room Temperature

18-28°C

not recommended

Refrigerated

 2-8°C

≤ 3 days

If separated within 3 hours

Frozen

≤-20 °C

≤ 3 weeks

If separated within 3 hours

Rejection Criteria

Gross Hemolysis

Result Reporting and Reference Values

Reported in mg/dL.

 

Sex

Age

Low

High

Male

0-6Y

30

65

6-15Y

38

75

15-18Y

30

63

18-150Y

40

59

Female

0-6Y

30

70

6-15Y

38

75

15-18Y

35

74

18-150Y

40

59

Reflex Testing

None

Limitations

  • Ortho reports a bias with the following:
    • Ascorbic acid at 3 mg/dL can cause a -9 mg/dL bias
    • Bronidox at 800 mg/dL can cause a +5 mg/dL bias
    • Dopamine at 3 mg/dL can cause a -10 mg/dL bias
    • Gentisic Acid at 5 mg/dL can cause a -10 mg/dL bias
    • N-acetylcysteine at 10 mg/dL can cause a -6 mg/dL bias
  • Ortho reports no significant effect with the following:  
      • Bilirubin (Bc) up to 10 mg/dL
      • Bilirubin (Bu) up to 40 mg/dL
      • Hemoglobin up to 400 mg/dL
      • Lipemia up to 800 mg/dL
  • The following interpretive data is visible to the practitioner
    • Therapeutic levels of Gentisic acid and N-acetylcysteine create negative biases

Useful For

High Density Lipoprotein (HDL) cholesterol is used to evaluate the risk of developing coronary heart disease (CHD).  The risk of CHD increases with lower HDL cholesterol concentrations.