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HelpTest Code C Blood Blood Culture
Methodology
Blood Culture automatically monitored via BacT/Alert Microbial Detection System.
Blood Culture ID Panel (multiplexed PCR) performed on BioFire FilmArray System.
AKA (Description)
Culture Blood
Specimen Requirement
For adult and pediatric patients > 80 lbs:
15 – 20 mL of blood is collected and distributed between the aerobic (FAN) and anaerobic (ANA) bottle. If less than 20 mL is collected, then 10 mL is inoculated in the aerobic bottle and the remaining into the anaerobic bottle.
For pediatric patients 30 – 80 lbs:
5 – 10 mL of blood is collected and injected into the aerobic bottle (FAN).
For pediatric patients ? 4 y/o:
No more than 4 mL of blood is collected and injected into the pediatric bottle (PFP).
Min. Volume
Aerobic bottle (FAN): 10 mL
Anaerobic bottle (ANA): 5 mL
Pediatric bottle (PF): 0.5 mL
Special Instructions
BacT/Alert blood culture bottles will be put on the BacT/Alert 3D to be continuously monitored for growth.
Non-BacT/Alert blood culture bottles will be manually checked twice a day for growth.
Test Included
Negative Blood Culture:
Culture
Positive Blood Culture:
Gram Stain, Blood Culture ID Panel (PCR), Culture, and Sensitivities (if applicable)
TAT
Negative Blood Culture
Final (automated): 5 days
Positive Blood Culture upon positive alert
Gram stain: 1 hour
PCR: 2 hours
Culture: 5 days for final